The film, which stars James Dornan and Dakota Johnson as the libidinous couple, far outpaced the No. 2 release, "Kingsman: The Secret Service," which took in $35.6 million from Friday through Sunday, according to studio estimates.
Third place on the US Presidents' Day holiday weekend went to the family-friendly "The SpongeBob Movie: Sponge Out of Water," based on the popular television show about a talking animated sponge, which sold $30.5 million in tickets at US and Canadian theaters.
"Fifty Shades" got a boost from a Valentine's Day opening after it was released earlier in the week in 57 foreign markets. It took in an additional $158 million overseas, for a global total of $240 million.
Early US screenings on Thursday night brought in $8.6 million, setting the scene for a record-breaking opening for any Presidents' Day weekend.
Nick Carpou, president of domestic distribution for Universal Pictures, the Comcast Corp. Unit that released the film, said a combination of Valentine's Day, a long holiday weekend and intense interest from smaller and mid-sized markets all helped drive the record numbers.
Pre-sale figures in southern states such as Mississippi and Kentucky reflected especially high interest. Sales in the northeast, being pummeled by another big winter storm, as well as in the south-central regions of the country, exceeded what might have been expected, Carpou said.
"Kingsman," an adaptation of a popular comic series starring Samuel L. Jackson and Colin Firth about a spy agency's training program and a global threat by a tech genius, received solid reviews and exceeded industry forecasts.
Rounding out the top five, the box office hit "American Sniper" took in $16.4 million, bringing its domestic haul to just over $304 million and making it one of the biggest hits of 2014-15. The Clint Eastwood-directed film is nominated for six Oscars, including one for Bradley Cooper as best actor.
The sci-fi film "Jupiter Ascending" was fifth with $9.4 million.
"Kingsman: The Secret Service" was released by 20th Century Fox, a unit of 21st Century Fox. "The SpongeBob Movie" was distributed by Paramount Pictures, a unit of Viacom Inc . "American Sniper" and "Jupiter Ascending" were released by Warner Brothers, a unit of Time Warner Inc. —Reuters
